Genome-wide analysis of astrocyte XBP1 activation and regulation of transcriptional programs in CNS cells during EAE.

We report XBP1 activation and regulation of pro-inflammatory signaling in astrocytes, microglia, and CNS-recruited pro-inflammatory monocytes during EAE. Overall design: Analysis of RNA expression in astrocytes, microglia, and monocytes sorted by flow cytometry. Mice transduced with astrocyte-targeting lentiviruses encoding non-targeting or Xbp1-targeting shRNAs.

本研究报道了实验性自身免疫性脑脊髓炎（EAE）进程中，星形胶质细胞（astrocytes）、小胶质细胞（microglia）以及中枢神经系统（CNS）募集的促炎性单核细胞内X盒结合蛋白1（XBP1）的激活情况，及其对促炎信号通路的调控作用。实验设计概述：通过流式细胞术（flow cytometry）分选出星形胶质细胞、小胶质细胞与单核细胞，对其RNA表达水平进行分析。实验对象为经靶向星形胶质细胞的慢病毒转导的小鼠，该慢病毒携带非靶向或靶向Xbp1的短发夹RNA（shRNAs）。