Sequence variations in MLS cell lines.

All variations are single nucleotide polymorphisms.1Genomic location at respective chromosome using hg19 genome sequence.2Codon change within the reading frame of respective gene.3Amino acid change due to the observed polymorphism. Both detected polymorphisms are normal and commonly occurring variants.4Cosmic annotated mutations with corresponding ID are indicated.5Variant observed. For example, for MLS1765-92 and TP53 (Het, G/C, C) data indicate that the observed variation (“C”) is heterozygote, while the reference nucleotide is “G”. Note that the codon change for TP53 is CCC>CGC, since the gene is located at the minus DNA strand. Het, heterozygote; Hom, homozygote.Sequence variations in MLS cell lines.