Sequence variations in MLS cell lines.

All variations are single nucleotide polymorphisms.1Genomic location at respective chromosome using hg19 genome sequence.2Codon change within the reading frame of respective gene.3Amino acid change due to the observed polymorphism. Both detected polymorphisms are normal and commonly occurring variants.4Cosmic annotated mutations with corresponding ID are indicated.5Variant observed. For example, for MLS1765-92 and TP53 (Het, G/C, C) data indicate that the observed variation (“C”) is heterozygote, while the reference nucleotide is “G”. Note that the codon change for TP53 is CCC>CGC, since the gene is located at the minus DNA strand. Het, heterozygote; Hom, homozygote.Sequence variations in MLS cell lines.所有变异均为单核苷酸多态性（single nucleotide polymorphisms）。 ¹ 基于hg19基因组序列的对应染色体基因组定位信息。 ² 对应基因可读框内的密码子改变情况。 ³ 由该多态性导致的氨基酸改变。本次检测到的两种多态性均为正常且常见的变异类型。 ⁴ 标注了带有对应ID的Cosmic注释突变。 ⁵ 观测到的变异。例如，针对MLS1765-92与TP53（Het, G/C, C）的数据集，其含义为：观测到的变异碱基为"C"，属于杂合子（heterozygote），参考碱基为"G"。需注意，由于该基因位于DNA负链上，TP53的密码子改变为CCC>CGC。Het为杂合子的缩写；Hom为纯合子（homozygote）的缩写。 MLS细胞系的序列变异情况。