Identification of genes involved with P. aeruginosa biofilm antibiotic resistance by microarray analysis. Pseudomonas aeruginosa

Microarray analysis was used to identify changes in the level of transcription of genes in P. aeruginosa drip flow biofilms in response to ciprofloxacin and tobramycin exposure. This data was evaluated and used to select strains that carry transposon mutations in genes that might play a role in antibiotic tolerance of biofilms. The strains were evaluated for defects in biofilm tolerance. Overall design: Four drip flow biofilm conditions with three replicates each: (1) baseline controls at 72 hours, (2) tobramycin treated for 12 hours past baseline, (3) ciprofloxacin treated for 12 hrs past baseline, and (4) no treatment for 12 hrs past baseline.

本研究采用微阵列分析（microarray analysis），旨在探究铜绿假单胞菌（P. aeruginosa）滴流生物膜（drip flow biofilms）在暴露于环丙沙星（ciprofloxacin）与妥布霉素（tobramycin）后，其基因转录水平的变化。经对该数据集进行分析评估，筛选出在可能参与生物膜抗生素耐受的基因中携带转座子突变（transposon mutations）的菌株，并针对这些菌株的生物膜耐受缺陷情况开展了评估。整体实验设计如下：共设置4组滴流生物膜实验条件，每组均设置3次生物学重复：1. 72小时基线对照组；2. 基线培养至72小时后，采用妥布霉素处理12小时；3. 基线培养至72小时后，采用环丙沙星处理12小时；4. 基线培养至72小时后，不施加药物继续培养12小时。