Expression data from D.melanogaster larvae with a constitutively active Imd in the fat body

Activation of innate immune responses in the Drosophila larval fat body affects the physiological host responses. In order to characterize the effect of the activated immune responses in the fat body on the Drosophila, we used whole-genome microarray analysis and found that activation of the immune deficieny pathway (Imd) in the fat body alters the transcriptional profiles of Drosophila larvae. As we expected many of genes involved in regulation of antimicrobial peptides were upregulated in the larvae with elevated Imd activity in the fat body. Notably, we found activatioan of Imd in the fat body negatively affects expression of genes involved in glycolysis, energy production and insulin signaling pathway. Overall, our analysis showed that activation of innate immune signaling in the larval fat body significantly affects cellular pathways that regulate metabolism. Genotype control larvae and larvae with a constitutively active Imd in the fat body were raised on regular food until they reached third instar larvae stage. Whole larvae were used for RNA extraction and RNA was hybridized to Affymetrix GeneChip®Drosophila Genome 2.0 arrays in order to investigate the effects of activation of innate immune responses in the fat body on the transcriptional profile of Drosophila larvae.

果蝇幼虫脂肪体的先天免疫应答激活，可影响宿主的生理应答反应。为阐明脂肪体中激活的免疫应答对果蝇的调控作用，我们采用全基因组微阵列分析技术，发现脂肪体中免疫缺陷通路（Immune Deficiency, Imd）的激活会改变果蝇幼虫的转录谱。正如预期，在脂肪体Imd活性升高的幼虫中，诸多参与抗菌肽调控的基因均出现上调表达。值得注意的是，我们发现脂肪体中Imd通路的激活会负向调控糖酵解、能量产生以及胰岛素信号通路相关基因的表达。综上，本研究分析表明，果蝇幼虫脂肪体的先天免疫信号激活，会显著影响调控代谢的细胞通路。本研究将基因型对照幼虫以及脂肪体中Imd通路组成型激活的幼虫，在常规培养基中饲养至三龄幼虫阶段。收集完整幼虫进行RNA提取，并将RNA与Affymetrix GeneChip®果蝇基因组2.0芯片进行杂交，以此探究脂肪体先天免疫应答激活对果蝇幼虫转录谱的影响。