Results of the gene ontology analysis.

In this study, an extensive analysis of microsatellite markers (Single Tandem Repeats—STRs) in Penaeus vannamei was conducted at an advanced level. The markers were thoroughly examined, characterized, and specific markers located within coding regions were identified. Out of a total of 306 STRs, 117 were classified as perfect markers based on their single repeat motif. Among these perfect markers, 62 were found to be associated with predicted coding genes (mRNA), which were involved in various functions such as binding, catalytic activity, ATP-dependent activity, transcription, structural and molecular regulation. To validate the accuracy of the findings, a sample of nine markers was subjected to in vitro testing, which confirmed the presence of polymorphisms within the population. These results suggest the existence of different protein isoforms within the population, indicating the potential of these markers for application in both population and phenotype-genotype association studies. This innovative approach opens up new possibilities for investigating the impact of genomic plasticity in populations of P. vannamei.本研究针对凡纳滨对虾（Penaeus vannamei）中的微卫星标记（microsatellite markers），即单核苷酸串联重复序列（Single Tandem Repeats，简称STRs），开展了系统性的高级别分析。研究团队对上述标记进行了全面检测与特征解析，并筛选出位于编码区内的特异性标记。在总计306个STRs中，117个依据其单一重复基序被归类为完美型标记。在这些完美型标记中，有62个与预测编码基因（mRNA）相关联，这些基因参与结合、催化活性、ATP依赖活性、转录调控以及结构与分子调控等多种生物学功能。为验证研究结果的准确性，研究人员选取9个标记样本开展体外实验，证实该种群中存在多态性。上述结果表明该种群内存在不同的蛋白质同工型，提示此类标记在群体研究及表型-基因型关联研究中具备应用潜力。本研究所采用的创新方法为探究凡纳滨对虾种群的基因组可塑性影响开辟了全新路径。