Gene expression data of interleukin-7-dependent and BCR-ABL1-transformed pre-B cells

This analysis focused on identifying factors that protect pre-B cells against DNA double strand break (DSB)-mediated DNA damage stress during pre-B cell differentiation. Differentiation of pre-B cells including immunoglobulin light chain gene recombination were performed by withdrawal of interleukin-7 (IL-7) from IL-7-dependent murine pre-B cells or by inhibition of the BCR-ABL1 kinase activity in BCR-ABL1-transformed pre-B cells. The BCR-ABL1 kinase inhibitor STI571 (Imatinib) was used for the inhibition of BCR-ABL1. IL-7-dependent murine pre-B cells were either cultured in IL-7 (10 ng/ml) or induced to differentiate by withdrawal of IL-7. BCR-ABL1-transformed pre-B cells were either treated with 10 µM STI571 (in absence or presence of 10 ng/ml IL-7) for 16 hours or cultured without STI571. Three samples for each condition were processed.

本研究旨在鉴定前B细胞（pre-B cell）分化过程中，可保护其免受DNA双链断裂（DNA double strand break, DSB）介导的DNA损伤应激的保护因子。前B细胞的分化（包括免疫球蛋白轻链基因重排）可通过两种方式诱导：一是从依赖白细胞介素7（interleukin-7, IL-7）的小鼠前B细胞中撤除IL-7；二是抑制经BCR-ABL1转化的前B细胞的BCR-ABL1激酶活性。本研究采用BCR-ABL1激酶抑制剂STI571（伊马替尼，Imatinib）来抑制BCR-ABL1活性。依赖IL-7的小鼠前B细胞可分为两组培养：一组在含10 ng/ml IL-7的培养基中培养，另一组通过撤除IL-7诱导分化。经BCR-ABL1转化的前B细胞则分为三组处理：一组用10 µM STI571处理16小时（培养基中可含或不含10 ng/ml IL-7），另一组不添加STI571进行培养。每种处理条件均设置3个生物学重复样本。