Hellbender skin transcriptome

In this study, we explored the efficacy of Bd vaccination on hellbender salamanders and functionally characterized host, host-associated microbiome, and pathogen throughout immunization and infection. We compared the function of hellbender skin, the skin microbiome, and Bd with a metatranscriptome approach at four experimental time points, (i) pre-vaccination and pre-infection, (ii) post-vaccination and pre-infection, (iii) early infection, and (iv) late infection.本研究探究了蛙壶菌（Bd）疫苗接种对美洲隐鳃鲵（hellbender salamander）的效力，并在免疫与感染全过程中，对宿主、宿主相关微生物组以及病原体开展功能表征。本研究采用宏转录组（metatranscriptome）分析方法，在四个实验时间点对美洲隐鳃鲵的皮肤功能、皮肤微生物组以及蛙壶菌的功能进行对比分析：(i) 疫苗接种前与感染前，(ii) 疫苗接种后且感染前，(iii) 感染早期，(iv) 感染晚期。