Microarray analysis of mRNA and long noncoding RNA expression profiles between multiple myeloma patients and control

To further investigate the differential expression gene of multiple myeloma.Three multiple myeloma samples and three normal samples were used for microarray. Bone marrow CD138+ cells were sorted from multiple myeloma patients and normal individuals. Samples were treated with TRIzol and RNA extracted.Gene expression profiling was carried out in three multiple myeloma patients and three controls.

为深入探究多发性骨髓瘤的差异表达基因，本研究采用3份多发性骨髓瘤样本与3份正常样本进行基因芯片（microarray）检测。实验人员从多发性骨髓瘤患者及正常个体的骨髓中分选CD138阳性细胞，随后使用TRIzol试剂处理样本并提取RNA，最终完成3例多发性骨髓瘤患者及3例对照个体的基因表达谱分析。