Genetic diversity in migratory bats: results from RADseq data for three tree bat species at an Ohio windfarm

Genetic analyses can identify the scale at which wildlife species are impacted by human activities, and provide demographic information useful for management. Here, we use thousands of nuclear DNA genetic loci to assess whether genetic structure occurs within Lasiurus cinereus (Hoary Bat), L. borealis (Red Bat), and Lasionycteris noctivagans (Silver-Haired Bat) bats found at a wind turbine site in Ohio, and to also estimate demographic parameters in each of these three groups. Our specific goals are to: 1) demonstrate the feasibility of isolating RADseq loci from these tree bat species, 2) test for genetic structure within each species, including any structure that may be associated with time (migration period), and 3) use coalescent-based modeling approaches to estimate genetically-effective population sizes and patterns of population size changes over evolutionary timescales. Thousands of loci were successfully genotyped for each species, demonstrating the value of RADseq for generati...

遗传分析可明确野生动物类群受人类活动影响的程度，并可为物种管理提供实用的种群动态信息。本研究利用数千个核DNA遗传位点，对美国俄亥俄州某风电场区域内采集的灰毛蝠（*Lasiurus cinereus*, Hoary Bat）、红蝙蝠（*L. borealis*, Red Bat）以及银毛蝠（*Lasionycteris noctivagans*, Silver-Haired Bat）开展遗传结构评估，并估算这三个类群各自的种群动态参数。本研究的具体目标包括：1）验证从这类树栖蝙蝠类群中获取RADseq（限制性位点相关DNA测序，Restriction-site Associated DNA sequencing）位点的可行性；2）检测各物种种内的遗传结构，包括与时间（迁徙周期）相关的遗传结构；3）采用基于溯祖理论的建模方法，估算有效种群大小以及演化时间尺度下的种群大小变化模式。本研究已成功为每个物种完成数千个位点的基因分型，证实了RADseq技术在...