Gene Expression of larval diapause in Wyeomyia. Gene Expression of larval diapause in Wyeomyia

This study measured the differential expression of genes from two populations of Wyeomyia smithii in the USA : KC in Maine and PB in New Jersey. The study reveals differential gene expression (DGE) in diapausing and developing larvae using microarrays. It identified the temporal and developmental pattern of DGE by showing the combined differential effects of length of day and time of day overlaid in a single plot illustrating DGE from two separate, comparative arrays. The study identified select hormonal and cell-signaling pathways underlying this pattern. This GEO record is for a full factorial comparison (1) between the two larval populations (KC, PB) and (2) reared under two photoperiods (SD, LD) for 13-14 days (Short-Day diapausing larvae L:D = 11:13 hours; Long-Day developing larvae L:D = 14.5:9.5 hours) and (3) sampled either during the day at 10-11h after lights on (ZT10) or at night 22-23h after lights-on (ZT22). Overall design: We compared the gene expression patterns (EXP) for the two populations: EXP1 = LD vs SD, EXP2 = ZT10 vs ZT22 using four biological replicates for each treatement and population including balanced dye-swaps. All probes including random probes were quantile-normalized across chips, subarrays, samples and replicates.

本研究针对美国两个种群的史密斯氏斑蚊（Wyeomyia smithii）开展基因差异表达分析，这两个种群分别为来自缅因州的KC种群与新泽西州的PB种群。本研究借助基因芯片（microarrays）技术，分析了滞育期与发育期中幼虫的基因差异表达（Differential Gene Expression, DGE）情况。通过将昼长与昼夜时间的联合差异效应整合至单张可视化图表中，该研究阐明了DGE的时序与发育模式，该图表展示了两组独立对照芯片的DGE结果。本研究还筛选出了调控该表达模式的关键激素与细胞信号通路。本GEO（Gene Expression Omnibus，基因表达综合数据库）记录对应一项全因子比较实验，实验包含三个维度：(1) 两个幼虫种群（KC、PB）间的比较；(2) 分别在短日照（SD，光周期L:D=11:13小时，对应滞育幼虫）与长日照（LD，光周期L:D=14.5:9.5小时，对应发育幼虫）条件下饲养13-14天的幼虫；(3) 分别在光照开启后10-11小时（ZT10，昼间）与光照开启后22-23小时（ZT22，夜间）进行采样。整体实验设计：我们对两个种群的基因表达模式（EXP）进行了比较，其中EXP1为长日照组与短日照组的比较，EXP2为ZT10组与ZT22组的比较；每个处理组与种群均设置4次生物学重复，并设置了平衡的染料互换（dye-swaps）实验。所有探针（包括随机探针）均在芯片、亚阵列、样本与重复间进行了分位数归一化（quantile-normalized）处理。