gene expression of CTCF-depleted mouse oocyte. Mus musculus

CTCF is a multifunctional nuclear factor involved in epigenetic regulation. We have used transgenic RNA interference to deplete maternal stores of CTCF from growing mouse oocytes, and identified the potential target genes Overall design: We profiled the global gene expression in the wild type and CTCF-depleted GV oocytes, and identified a set of target genes.

CTCF是一种多功能核因子，参与表观遗传调控（epigenetic regulation）。本研究通过转基因RNA干扰技术，从发育中的小鼠卵母细胞中耗竭母源性CTCF储备，并鉴定出其潜在靶基因。整体实验设计：我们对野生型与CTCF耗竭的生发泡（germinal vesicle, GV）卵母细胞开展全局基因表达谱分析，进而鉴定出一组靶基因。