Expression profile of Pseudomonas aeruginosa ?gcsR PAO1 using RNA-Seq

The TyrR-like enhancer-binding protein GcsR (or PA2449) was shown to regulate the expression of genes required for glycine metabolism. In order to define the regulon of GcsR we compared the transcriptome of a gcsR deletion mutant of P. aeruginosa PAO1 with that of the wild-type using RNA-Seq. Overall design: Strains were grown under glycine rich conditions (peptone broth) and their transcriptomes were compared using RNA-Seq.

类TyrR增强子结合蛋白GcsR（又名PA2449）已被证实可调控甘氨酸代谢相关基因的表达。为明确GcsR的调控子（regulon），本研究采用RNA-Seq技术，对比了铜绿假单胞菌（P. aeruginosa）PAO1的gcsR缺失突变株与野生型菌株的转录组。实验设计概述：菌株于富甘氨酸培养条件（蛋白胨肉汤培养基）中培养，随后通过RNA-Seq技术比对二者的转录组。