m6A RNA methylation of major satellite repeat transcripts facilitates chromatin association and RNA:DNA hybrid formation in mouse heterochromatin

m6A RNA methylation of major satellite repeat transcripts facilitates chromatin association and RNA:DNA hybrid formation in mouse heterochromatin Overall design: Analysis of m6A-modified transcripts in mouse embryonic stem cells (mutants for Mettl3, Mettl14 and corresponding wild types) Analysis of gene expression in mouse embryonic stem cells (mutants for Mettl3, Mettl14 and corresponding wild types)

小鼠异染色质中主要卫星重复序列转录本的m6A RNA甲基化可促进染色质结合与RNA:DNA杂交结构形成。总体实验设计：1. 对Mettl3、Mettl14敲除突变型小鼠胚胎干细胞及其对应野生型细胞的m6A修饰转录本进行分析；2. 对上述基因型小鼠胚胎干细胞的基因表达水平进行分析。