Cofactor specificity of the tetrameric 6-phosphogluconate dehydrogenase from Gluconobacter oxydans: Studies of wild-type and mutants forms.

Here we present the results regarding the characterization of the kinetics parameters for NAD and NADP of the 6-phosphogluconate dehydrogenase from Gluconobacter oxydans at different concentrations of 6PG. The raw data of kinetic parameters for NAD or NADP at different 6PG concentrations are represented in kcat and KM, with the units s-1 and µM, respectively. Al the rest values as an efficient constant are represented in s-1µM-1. Also, we included the kinetic characterizations of the mutants Go6PGDH-R31L, Go6PGDH-R31V, and Go6PGDH-D30N. The CSV files contain four columns. 1. NAD or NADP concentration in µM; 2. enzyme activity in U/mg; 3. Standard deviation and 4. The number of independent experiments.本研究呈现了氧化葡糖杆菌（Gluconobacter oxydans）来源的6-磷酸葡糖酸脱氢酶（6-phosphogluconate dehydrogenase）在不同6-磷酸葡糖酸（6PG）浓度下，针对烟酰胺腺嘌呤二核苷酸（NAD）与烟酰胺腺嘌呤二核苷酸磷酸（NADP）的动力学参数表征相关结果。针对不同6PG浓度下NAD或NADP的动力学参数原始数据以催化常数（kcat）与米氏常数（KM）形式呈现，二者的单位分别为s⁻¹与µM。其余所有效率常数类数值的单位均为s⁻¹·µM⁻¹。此外，本研究还纳入了突变体Go6PGDH-R31L、Go6PGDH-R31V以及Go6PGDH-D30N的动力学表征结果。逗号分隔值（CSV）文件包含四列数据：1. NAD或NADP浓度（单位：µM）；2. 酶比活（单位：U/mg）；3. 标准偏差；4. 独立实验次数。