Raw diffraction images of [NiFe]-hydrogenase maturation factor HypD from Aquifex aeolicus (C360S mutant)

HypD is one of the maturation factors of [NiFe]-hydrogenase and can form a complex with other maturation factors (Muraki <em>et al.</em>, 2019). We used datasets to investigate the protocol of detecting polymorphs using Hierarchical clustering (Acta D., submitted). All diffraction datasets were collected at BL45XU, SPring-8, using an automated data collection system <em>ZOO</em>. From six crystals, datasets were collected from each one using a continuous helical scan scheme for 360º oscillation with the following experimental parameters; Beam size: 20 µm × 20 µm, Wavelength: 1.0000 Å, Total dose/crystal: 10 MGy, Detector: EIGER X 9M (DECTRIS Co. Ltd.). All crystals belonged to space group P212121 with unit cell parameters roughly corresponding to a=60.0, b=62.6, c=97.6 Å.HypD是[NiFe]-氢化酶（[NiFe]-hydrogenase）的成熟因子之一，可与其他成熟因子形成复合物（Muraki等，2019）。本数据集用于探究基于层级聚类（Hierarchical clustering）检测多晶型物的实验方案（Acta D.，已投稿）。所有衍射数据集均采集于SPring-8同步辐射光源的BL45XU光束线，使用自动化数据采集系统ZOO完成采集。从6颗晶体中，每颗均采用连续螺旋扫描方案采集数据集，振荡范围为360º，实验参数如下：光束尺寸为20 µm × 20 µm，波长为1.0000 Å，单晶体总辐照剂量为10 MGy，探测器为EIGER X 9M（DECTRIS有限公司）。所有晶体均属于P212121空间群，晶胞参数大致为a=60.0、b=62.6、c=97.6 Å。