Tolerance mechanisms towards high-temperature and -acid stress conditions during itaconic acid biosynthesis in Aspergillus terreus. Aspergillus terreus

A. terreus LYT10 is an industrial strain for itaconic acid production, in which the biosynthesis of itaconic acid and glucose conversion rate were affected by temperature and initial concentration of itaconic acid in industrial production. RNA-seq was used to identify the key regulators related to tolerance mechanism toward various stress conditions. Overall design: A total of 4 samples were analzyed. The mycelia cultivated in itaconic acid production medium (IPM) on a rotary shaker at 220 rpm and 37°C for 36 h was considered as a reference. IPMs with 5 g/L and 40 g/L itaconic acid (pH 3.25) were used for high-acid culture respectively. High-temperature condition was possesed at 42°C for 36 h in IPM.

土曲霉（Aspergillus terreus）LYT10是一株用于衣康酸生产的工业菌株。工业生产场景下，该菌株的衣康酸生物合成过程与葡萄糖转化率均受温度及衣康酸初始浓度的调控。本研究采用RNA测序（RNA-seq）技术，筛选与多重胁迫耐受机制相关的关键调控因子。实验设计概况：共计4个样本参与分析。以在衣康酸生产培养基（IPM）中、220 rpm摇床、37℃条件下培养36 h获得的菌丝体作为对照样本；分别采用添加5 g/L和40 g/L衣康酸（pH 3.25）的IPM进行高酸培养；高温培养组为在IPM中于42℃条件下培养36 h。