Supplementary Material for: Clinical Protocols for the Evaluation of Rod Function

This work presents a quick clinical protocol for dark-adapted chromatic (DAC) perimetry as well as a novel clinical tool, scotopic chromatic pupil campimetry (CPC). The goal of the study was to explore the applicability of these methods in a clinical setting, their test-retest repeatability, and the congruence of the results. Local rod sensitivity was assessed at 36 locations within 30° eccentricity of the visual field in 15 healthy subjects (mean age 43 ± 16 years; 7 females and 8 males) with DAC perimetry (red and cyan stimuli) and CPC 2 times in repeated measurements. The duration of individual measurements was 370 ± 5 s for CPC and 366 ± 62 s for DAC perimetry. The intraclass correlation (ICC) coefficient was 0.53 for DAC perimetry cyan stimuli, 0.67 for red stimuli, and 0.93 for CPC. However, the spatial resolution of CPC was substantially smaller than in DAC perimetry. We did not find a correlation of DAC perimetry and CPC measurements on the global or the local level. In comparison to DAC perimetry, CPC shows a superior intervisit repeatability in detecting functional changes in the rod population in an objective way with lower spatial resolution. Our results also indicate that these 2 methods measure the rod function in different ways and could thus constitute complementary scotopic functional diagnostics.

本研究提出了一种快捷的暗适应色觉视野检查法（dark-adapted chromatic perimetry, DAC）临床方案，以及一款新型临床工具——暗适应色觉瞳孔视野检查法（scotopic chromatic pupil campimetry, CPC）。本研究旨在探索这两种方法在临床场景中的适用性、重测信度，以及检测结果的一致性。研究纳入15名健康受试者（平均年龄43±16岁，其中女性7名、男性8名），采用DAC视野检查法（红色、青色刺激）与CPC分别对视野30°偏心范围内的36个位点进行视杆细胞敏感度检测，且所有测量均重复进行2次。单次测量时长方面，CPC为370±5秒，DAC视野检查法为366±62秒。组内相关系数（intraclass correlation, ICC）结果显示，DAC视野检查法的青色刺激组ICC值为0.53，红色刺激组为0.67，CPC组则为0.93。但CPC的空间分辨率显著低于DAC视野检查法。本研究未发现DAC视野检查法与CPC的检测结果在全局或局部层面存在相关性。相较于DAC视野检查法，CPC在以客观方式检测视杆细胞群体功能变化时，具有更优的跨访视重测信度，但空间分辨率更低。本研究结果同时表明，这两种方法通过不同路径检测视杆细胞功能，因此可作为互补性的暗适应功能诊断手段。