Single-cell RNA-sequencing of cells from spinal cord central canal region of young adult and aged mice

The spinal cord neural stem cell potential is contained within the ependymal cells lining the central canal. This neural stem cell potential is known to decline with age in the mouse. Here, we microdissected and dissociated into single cells the central canal region from the spinal cord of 4 young adult (3-to-4-month old) and 4 aged (18-to-19-month old) C57BL/6J mice to profile the transcriptomes of cells in and around the central canal using 10x Genomics technology.

脊髓神经干细胞潜能定位于衬覆中央管的室管膜细胞内。已知该神经干细胞潜能在小鼠体内随衰老进程而逐渐衰退。本研究对4只年轻成年（3至4月龄）及4只老年（18至19月龄）C57BL/6J小鼠的脊髓中央管区域进行显微解剖并解离为单细胞，采用10x Genomics技术对中央管内及周边细胞的转录组进行分析。