Florida reef tract seawater microbial communities during SCTLD outbreak

The Florida Reef Tract is currently experiencing a widespread outbreak of Stony Coral Tissue Loss Disease (SCTLD). At the time of sample collection in 2019, there were only a couple of areas that had yet to show outbreaks of this disease, which included the Marquesas and Dry Tortugas National Park. During June 2019, we sampled seawater in 2L duplicates along the entire reef tract, from near Miami, Florida to the Dry Tortugas National Park to understand how the seawater microbial communities shifted along a wide geographic range and in the face of differing levels of SCTLD prevalence. All sequence data represent the V4 regions of the 16S rRNA gene of bacteria and archaea that were sequenced using the Illumina Miseq 2x250 bp.

佛罗里达礁区带（Florida Reef Tract）目前正暴发大范围的石珊瑚组织脱落病（Stony Coral Tissue Loss Disease，SCTLD）疫情。2019年样本采集期间，该区域仅少数区域尚未出现该病暴发，其中包括马尔克斯群岛（Marquesas）与德赖托图格斯国家公园（Dry Tortugas National Park）。2019年6月，我们沿整个礁区带从佛罗里达迈阿密附近至德赖托图格斯国家公园，采集了2升重复海水样本，以探究海水微生物群落如何随广阔地理梯度以及不同SCTLD流行水平发生动态变化。所有测序数据均为采用Illumina MiSeq 2×250 bp测序平台获得的细菌与古菌16S rRNA基因V4区序列。