Data_Sheet_1_Red Carrot Cells Cultured in vitro Are Effective, Stable, and Safe Ingredients for Skin Care, Nutraceutical, and Food Applications.XLSX

Plant biomasses growing in bioreactor could be developed as production systems for cosmetic ingredients, nutraceuticals and food additives. We previously reported that the red carrot cell line R4G accumulates high levels of anthocyanins, which are potent antioxidants with multiple health-promoting properties. To investigate the industrial potential of this cell line in detail, we tested extract for antioxidant and anti-inflammatory activity in the mouse monocyte/macrophage cell-line J774A.1 and in reconstructed skin tissue models. We also compared the R4G extract to commercial carrot extracts in terms of stability and metabolomic profiles. We found that the R4G extract have potent antioxidant and anti-inflammatory activities, protecting mammalian cells from the oxidative stress triggered by exposure to bacterial lipopolysaccharides and H2O2. The extract also inhibited the nuclear translocation of NF-κB in an epidermal skin model, and induced the expression of VEGF-A to promote the microcirculation in a dermal microtissue model. The anthocyanins extracted from R4G cells were significantly more stable than those found in natural red carrot extracts. Finally, we showed that R4G extract has similar metabolomic profile of natural extracts by using a combination of targeted and untargeted metabolomics analysis, demonstrating the safety of R4G carrot cells for applications in the nutraceutical and food/feed industries.

生长于生物反应器(bioreactor)中的植物生物质，可被开发为化妆品原料、营养保健品(nutraceuticals)及食品添加剂的生产体系。本课题组此前已报道，红胡萝卜细胞系R4G可积累高水平的花青素(anthocyanins)，这类物质属于强效抗氧化剂，具备多种健康促进特性。为深入探究该细胞系的工业应用潜力，我们分别在小鼠单核/巨噬细胞系J774A.1以及重建皮肤组织模型中，测试了其提取物的抗氧化与抗炎活性。我们还将R4G提取物与商业胡萝卜提取物在稳定性及代谢组学特征(metabolomic profiles)方面进行了对比。研究结果显示，R4G提取物具备强效抗氧化与抗炎活性，可保护哺乳动物细胞免受细菌脂多糖(lipopolysaccharides)与过氧化氢(H2O2)暴露所诱发的氧化应激损伤。该提取物还可抑制表皮皮肤模型中核因子κB(NF-κB)的核转位，并在真皮微组织模型中诱导血管内皮生长因子A(VEGF-A)的表达，以促进微循环。从R4G细胞中提取的花青素，其稳定性显著优于天然红胡萝卜提取物中的花青素。最后，通过结合靶向代谢组学与非靶向代谢组学分析，我们证实R4G提取物与天然提取物的代谢组学特征高度相似，证明了R4G胡萝卜细胞应用于营养保健品及食品/饲料工业的安全性。