Expression data from human PDAC-derived cancer-associated fibroblast (CAF) treated with isorhamnetin

Human pancreatic ductal adenocarcinoma (PDAC) derived cancer-associated fibroblast (CAF) treated or not with isorhamnetin We used microarrays to detail the global hallmarks, gene ontology, and KEGG pathway terms affected by isorhamnetin treatment of CAF derived from human PDAC PDAC derived CAFs were immortalized and treated with 20 µM isorhamnetin for 96 h, followed by total RNA extraction and hybridized on Applied Biosystems Clariom S array

本数据集的实验样本为经或未经异鼠李素（isorhamnetin）处理的人胰腺导管腺癌（PDAC）来源癌症相关成纤维细胞（CAF）。我们采用微阵列芯片，详细解析了异鼠李素处理人PDAC来源CAF所影响的全局特征、基因本体（GO）及KEGG通路术语。具体实验流程如下：将PDAC来源的CAF进行永生化，随后以20 μM异鼠李素处理96小时，之后提取总RNA并在应用生物系统（Applied Biosystems）Clariom S基因芯片上完成杂交。