RNA sequencing data describing transcriptional changes in aorta of ApoE-/- mice after alpha 7 nicotinic acetylcholine receptor (α7nAChR) stimulation.

This manuscript is a companion paper to Ulleryd M.U. et al (1). Data shown here include RNA sequencing data from whole aorta of ApoE-/- mice treated with the alpha 7 nicotinic acetylcholine receptor (α7nAChR) agonist AZ6983 for 8 weeks using subcutaneously implanted osmotic minipumps. 1. Ulleryd MA, Mjornstedt F, Panagaki D, Yang LJ, Engevall K, Gutierrez S, et al. Stimulation of alpha 7 nicotinic acetylcholine receptor (α7nAChR) inhibits atherosclerosis via immunomodulatory effects on myeloid cells Re-submitted. 2019. ApoE-/- mice were treated with alpha 7 nicotinic acetylcholine receptor (α7nAChR) agonist AZ6983 or vehicle for 8 weeks using subcutaneously implanted osmotic minipumps. RNA from whole aorta were extracted and used for RNA sequencing analysis. n=6 per group.

本手稿为Ulleryd M.U.等人（参考文献1）的配套研究论文。本次展示的数据源自经皮下植入渗透压微型泵（osmotic minipumps）持续给药8周的ApoE-/-小鼠全主动脉的RNA测序（RNA sequencing）数据，给药化合物为α7烟碱型乙酰胆碱受体（α7nAChR）激动剂AZ6983。
1. Ulleryd MA, Mjornstedt F, Panagaki D, Yang LJ, Engevall K, Gutierrez S, 等. α7烟碱型乙酰胆碱受体（α7nAChR）激动通过对髓系细胞的免疫调节作用抑制动脉粥样硬化[已重新投稿]. 2019.
本实验中，ApoE-/-小鼠经皮下植入的渗透压微型泵分别给予α7烟碱型乙酰胆碱受体（α7nAChR）激动剂AZ6983或溶媒对照，给药周期为8周。随后提取全主动脉总RNA并开展RNA测序分析，每组设置6个生物学重复（n=6）。