Expression profile of CXCR6 Hi T cells or CXCR6 Lo helper T cells in CD45Rb Hi tranfer model mice

To identify the role of chemokine receptor in inflammation of colon, we isolated CD3+CD4+ helper T cells harboring CXCR6 from colonic lamina propria of mice We used microarrays to identify the differentially expressed genes between CXCR6Hi Tcells and CXCR6Lo Tcells CXCR6 Hi or CXCR6 Lo T cells were isolated from colonic lamina propria for RNA extraction and hybridization on Affymetrix microarrays.

为探究趋化因子受体在结肠炎症中的作用机制，我们从小鼠结肠固有层中分离得到了表达CXCR6的CD3+CD4+辅助性T细胞。我们采用基因芯片（microarrays）技术，筛选CXCR6高表达T细胞与CXCR6低表达T细胞之间的差异表达基因。同时，我们从结肠固有层分离CXCR6高表达或低表达T细胞，用于RNA提取及在Affymetrix基因芯片上完成杂交实验。