List of oligos used for Cloning of caf1, lcrV and hsp70(II) genes in pET28a+ vector.

Homologous nucleotide sequences of caf1, lcrV and hsp70(II) in capital case and the engineered sequences (ata) at the 5′ ends are shown in small case. The in-frame initiator codon in the forward primer is shown in bold and the compatible restriction sites are underlined.List of oligos used for Cloning of caf1, lcrV and hsp70(II) genes in pET28a+ vector.本数据集展示了以大写字母形式呈现的caf1、lcrV及hsp70(II)同源核苷酸序列，5'端带有(ata)的工程化序列则以小写字母标注。正向引物中的框内起始密码子以粗体标示，匹配性限制性酶切位点以下划线标注。以下为用于将caf1、lcrV及hsp70(II)基因克隆至pET28a+载体的寡核苷酸引物列表。