microRNA expression profiles in myocardium of diet-induced obesity rat

Diet induced obesity in rat was associated with myocardial dysfunction, hypertension and fibrosis. This study aimed to explore microRNA expression profiles in diet obesity-induced rat myocardium. Wistar rats were feed normal chow or high-fat diet for 20 weeks. After that, cardiac function was evaluated by echocardiography. Left ventricular myocardium was harvest to assess the extent of hypertension and fibrosis, meanwile, the left ventricular microRNA expression was analyzed using Agilent Rat miRNA microarray. Significant cardiac dysfunction, hypertension and fibrosis were found in diet-induced obesity rats as compared with normal diet rats. rno-miR-141-3p and rno-miR-144-3p were also significantly increased in myocardium of diet-induced obesity rat. These findings suggest that specific miRNA differences may contribute to the alteration in cardiac function, hypertension and fibrosis which responses to diet-induced obesity. Wistar rats were randomly divided into two groups, which feed normal chow or high-fat diet for 20 weeks, respectively. Left ventricular myocardium was harvest to detect microRNA expression using microarray. Furthermore, miRNA differences were analyzed by comparing the microRNA expression in myocardium between rats fed normal diet and rats fed high-fat diet.

饮食诱导的大鼠肥胖与心肌功能障碍、高血压及心肌纤维化密切相关。本研究旨在探究饮食诱导肥胖大鼠心肌组织中的微小RNA（microRNA）表达谱。将Wistar大鼠随机分为两组，分别饲喂正常饲料与高脂饲料，持续饲养20周。随后，通过超声心动图评估大鼠心脏功能；采集左心室心肌组织以评估高血压与纤维化程度，同时采用安捷伦大鼠miRNA芯片（Agilent Rat miRNA microarray）分析左心室心肌的微小RNA表达情况。与正常饲料组大鼠相比，饮食诱导肥胖大鼠出现了显著的心脏功能障碍、高血压及心肌纤维化，且其心肌组织中rno-miR-141-3p与rno-miR-144-3p的表达水平亦显著升高。上述研究结果表明，特定miRNA的表达差异可能参与介导饮食诱导肥胖所引发的心脏功能改变、高血压及心肌纤维化。本研究将Wistar大鼠随机分为两组，分别饲喂正常饲料与高脂饲料，持续20周；采集左心室心肌组织，通过芯片技术检测其miRNA表达水平，并通过对比两组大鼠的心肌miRNA表达谱，分析差异表达的miRNA。