Characterization of muscle growth and sarcomere branching in the striated musculature of C. elegans

This is a dataset of JG images generated as snapshots of confocal micrographs obtained using a Leica SP8 confocal microscope at 63X magnification using the Lightning deconvolution mode. The images are of C. elegans nematodes body wall (striated) muscles. The animals were raised under different conditions in order to elicit different types of muscle growth. The impermeable cuticle of animals were then removed using the freeze-crack method and the f-actin in their muscles were stained following standard techniques. In this case we used green phalloidin. WGA was also used in some instances to stain muscle membranes. Worms were processed, mounted, and imaged the same afternoon during any one session. Myocytes were identified using anatomical features and measured as described in the accompanying preprint: doi: https://doi.org/10.1101/2024.08.30.610496.本数据集包含JG图像，这些图像为使用徕卡（Leica）SP8共聚焦显微镜，以63倍放大倍率并开启Lightning去卷积模式采集得到的共聚焦显微照片快照。图像的拍摄对象为秀丽隐杆线虫（C. elegans）的体壁横纹肌。研究人员通过设置不同培养条件，以诱导产生不同类型的肌肉生长。随后采用冻裂法去除线虫的不透性角质层，并通过标准染色技术对肌肉中的丝状肌动蛋白（f-actin）进行标记。本次实验使用绿色鬼笔环肽完成染色，部分样本还使用了小麦胚芽凝集素（WGA）对肌肉细胞膜进行染色。所有线虫均在同一批次实验的当日下午完成样本处理、封片及成像操作。肌细胞通过解剖学特征进行识别，并按照配套预印本中描述的方法进行量化分析，预印本DOI链接：https://doi.org/10.1101/2024.08.30.610496。