Inline holographic microscopy through a fibre imaging bundle Fig. 2

This dataset and code provides an example for reconstructing inline holography images through a fibre imaging bundle. The sample is 5 micron diameter microspheres on a 1 mm thick glass slide. The slide was back illuminated by 450 nm LED light coupled through a multimode fibre with a 50 micron diameter core. The illumination fiber was approximately 15 mm behind the slide. The hologram was collected by a Fujikura FIGH-30-650S fibre bundle approximately 0.5 mm in front of the microspheres. At the proximal end, this was imaged onto a CMOS camera via a 20X objective and a tube lens, giving an effective pixel size of 0.44 microns.本数据集及配套代码提供了一个通过光纤成像束（fibre imaging bundle）重建同轴全息（inline holography）图像的示例。实验样品为置于1 mm厚玻璃载玻片上的直径5微米微球。该载玻片采用450 nm波长的LED光源进行背照照明，光源通过纤芯直径为50微米的多模光纤（multimode fibre）耦合，照明用光纤位于载玻片后方约15 mm处。全息图由Fujikura FIGH-30-650S型光纤成像束采集，该光纤束位于微球前方约0.5 mm位置。在光纤束的近端，通过20倍物镜与镜筒透镜将其成像至CMOS相机（CMOS camera），最终有效像素尺寸为0.44微米。