Characteristics of cells and spheroids used in this study.

Notes:
1. Larger with addition of MRC5 fibroblast cells;
2. Spheroid formation goes through observable changes from disc to sphere that take about 5 days;
3. Spheroids are small and have limited growth potential;
4. EF5 binding was only seen in MC and TF spheroids—hypoxia in spheroids was only observed centrally.
5. Although SQ20B and U87 TFs both underwent rapid transformation to spherical shape, central hypoxia and necrosis in the former limited any growth, so their size reached a maximum at about 6 days and then declined with time. U87 TF spheroids continued to grow and could reach 1.5 to 2 mm in diameter. NA means non-applicable, ND means not done.
Characteristics of cells and spheroids used in this study.

备注：
1. 样本体量更大，且添加了MRC5成纤维细胞（fibroblast）；
2. 细胞球体形成过程会经历从盘状到球状的可观测形态演变，该过程耗时约5天；
3. 所制备的细胞球体体积较小，且生长潜能有限；
4. EF5结合信号仅可在MC及TF细胞球体中被检测到，提示细胞球体的缺氧区域仅局限于中心部位；
5. 尽管SQ20B与U87 TF细胞球体均能快速完成向球状形态的转变，但前者的中心缺氧及坏死会抑制其生长，因此其体积约在6天时达到峰值，随后随时间推移逐渐缩小；而U87 TF细胞球体可持续生长，直径可达1.5至2毫米。NA代表不可用（non-applicable），ND代表未完成（not done）。

本研究所用细胞及细胞球体的特征