Gene expression profile at single cell level of non-myocytic cells of the heart (NCM) from TRPM4 WT and KO mice 24h or 72h after SHAM intervention or LAD ligation. Gene expression profile at single cell level of non-myocytic cells of the heart (NCM) from TRPM4 WT and KO mice 24h or 72h after SHAM intervention or LAD ligation

Many different cell types constitute the heterogenous group of non-cardiomyocytes (NCM) of the heart. We used single cell RNA sequencing (scRNA-seq) to analyze the diversity of NCM and especially how the absence of TRPM4 affects the inflammatory response post myocardial infarction. Overall design: NCMs were isolated from B6.Cg-Trpm4tm1.2-PG (KO) mice or WT mice hearts either 24h after SHAM intervention or LAD ligation or 72h after LAD ligation. The live NCMs were isolated by Fluorescence-activated cell sorting (FACS) according to the presence or absence of Calcein-AM and DAPI signal and the NCM of 4 mice per sample/condition were pooled and analyzed using scRNAseq.

心脏非心肌细胞（non-cardiomyocytes, NCM）是一类具有异质性的细胞群，由多种不同细胞类型构成。本研究采用单细胞RNA测序（single cell RNA sequencing, scRNA-seq）技术分析NCM的细胞多样性，重点探究TRPM4缺失对心肌梗死后炎症应答的影响。
实验设计概述：
分别从B6.Cg-Trpm4tm1.2-PG基因敲除（KO）小鼠与野生型（WT）小鼠心脏中分离NCM，样本采集的时间点包括：假手术（SHAM）干预后24小时、左前降支结扎（LAD ligation）后24小时，以及左前降支结扎后72小时。通过钙黄绿素-AM（Calcein-AM）与4',6-二脒基-2-苯基吲哚（DAPI）的信号有无，采用荧光激活细胞分选（Fluorescence-activated cell sorting, FACS）分离存活的NCM；每个样本/实验条件下，将4只小鼠的NCM混合后，利用scRNA-seq进行测序分析。