Targeting VRK2 Enhances the Efficacy of Anti-PD-1 Therapy in Hepatocellular Carcinoma by Reducing Direct Phosphorylation and Stability of MYC,CUT-TAG sequencing for MYC in hepatocellular carcinoma cells [CUT&Tag]

VRK2–MYC axis, a transcription-dependent regulatory axis, could be a prognostic indicator and potential therapeutic target in liver cancer. Overall design: Cleavage Under Targets and Tagmentation (CUT&TAG) sequencing was then performed on MYC in cells from different treatment groups,

VRK2-MYC轴作为一种转录依赖性调控轴，可作为肝癌的预后标志物与潜在治疗靶点。实验整体设计：随后对不同处理组细胞中的MYC开展了靶标切割与标签测序（Cleavage Under Targets and Tagmentation, CUT&TAG）。