mRNA and miRNA expression profiling of human neutrophils after single and sequential stimulation with PAF and fMLP

This dataset contains raw and processed mRNA and miRNA microarray data obtained from human neutrophils under basal conditions and after stimulation with platelet-activating factor (PAF) and N-formyl-Met-Leu-Phe (fMLP), applied as single or sequential stimuli. Neutrophils were isolated from peripheral blood of five healthy adult male donors and distributed into five experimental conditions: unstimulated control, PAF, fMLP, PAF followed by fMLP, and fMLP followed by PAF, totaling 25 samples. The dataset includes raw CEL files, processed expression matrices, sample metadata, and supplementary files related to differential expression analysis. Data normalization and preprocessing were performed using the Robust Multi-array Average (RMA) method. Differential expression analyses were carried out to compare the effects of single and sequential stimulation on neutrophil miRNA expression profiles. This deposit was created to provide public access to the data and analytical files associated with the dissertation study on neutrophil regulatory responses under distinct inflammatory stimulation conditions.本数据集收录了处于基础状态下，以及经血小板活化因子（platelet-activating factor, PAF）、N-甲酰甲硫氨酰亮氨酰苯丙氨酸（N-formyl-Met-Leu-Phe, fMLP）单独或序贯刺激后人中性粒细胞的原始与处理后的信使RNA（mRNA）及microRNA（miRNA）微阵列数据。中性粒细胞取自5名健康成年男性供者的外周血，被分为5组实验条件：未刺激对照组、PAF单独刺激组、fMLP单独刺激组、PAF序贯fMLP刺激组以及fMLP序贯PAF刺激组，共计25份样本。 本数据集包含原始CEL文件、处理后的表达矩阵、样本元数据以及与差异表达分析相关的补充文件。数据标准化与预处理采用稳健多阵列平均（Robust Multi-array Average, RMA）方法完成。本研究针对该数据集开展了差异表达分析，以对比单独刺激与序贯刺激对中性粒细胞miRNA表达谱的影响。 本数据存档旨在公开提供与该学位论文研究相关的数据及分析文件，该研究聚焦于不同炎症刺激条件下中性粒细胞的调控应答反应。