Non-culture-based studies of the appendiceal microbiota: a systematic review - Methods-Reporting-Checklist_FM

Aims: To review studies examining the appendiceal microbiota and microbial changes in acute appendicitis. Methods: After a systematic literature search, 11 studies examining the appendiceal microbiota (414 samples) using non-culture-based methods were included. Results: The appendiceal microbiota showed decreased α-diversity compared with fecal microbiota. Inflamed and uninflamed appendices showed differences in β-diversity, and there was an increased abundance of oral-associated bacteria in inflamed versus uninflamed appendices. Conclusion: The appendiceal microbiota exhibits lower α-diversity than the fecal microbiota, with an increased abundance of oral-associated bacteria. Compared with uninflamed appendices, the appendix microbiota in acute appendicitis also showed increased abundance of oral-associated bacteria, but no bacterial profile unique to either complicated or uncomplicated appendicitis was found.研究目的：系统回顾针对阑尾微生物群（appendiceal microbiota）及急性阑尾炎（acute appendicitis）中微生物群变化的相关研究。 研究方法：经系统性文献检索后，最终纳入11项采用非培养法（non-culture-based methods）检测阑尾微生物群的研究，共涉及414份样本。 研究结果：相较于粪便微生物群（fecal microbiota），阑尾微生物群的α多样性（α-diversity）显著降低。发炎阑尾与未发炎阑尾的β多样性（β-diversity）存在差异，且发炎阑尾中口腔相关细菌（oral-associated bacteria）的丰度高于未发炎阑尾。 研究结论：阑尾微生物群的α多样性低于粪便微生物群，且口腔相关细菌丰度升高。与未发炎阑尾相比，急性阑尾炎患者的阑尾微生物群同样表现出口腔相关细菌丰度升高的特征，但未发现复杂性阑尾炎与非复杂性阑尾炎各自特有的细菌谱。