Time-course transcriptome data of LED Lsa under LD24. Lactuca

Lettuce plants were grown in a closed cultivation system. Seeds were sown on a water-laden urethane sponge in a tray filled with water and incubated for a week under fluorescent light. After one week, seedlings were transplanted to the multistage hydroponic system. The light sources used were red, green and blue LEDs. Cultivation was performed using a Deep Flow Technique hydroponic system. We sampled the maximum leaves every 2 h for 2 d, starting at 13 d and ending 15 d after transplanting. The maximum leaves were immediately frozen in liquid nitrogen and stored at -80 degree. We prepared a RNA-Seq library and obtained the sequence read files using a HiSeq 2000 sequencer.本研究将生菜植株种植于封闭栽培系统中。将种子播种于盛水托盘内的含水聚氨酯海绵上，随后置于荧光灯下培养一周。培养一周后，将幼苗移栽至多阶水培系统中。所用光源为红、绿、蓝三色发光二极管（LED）。栽培采用深液流技术（Deep Flow Technique）水培系统。自移栽后第13天起至第15天止，连续2天每2小时采集一次最大叶片。采集的最大叶片立即置于液氮中冷冻，并保存于-80℃环境中。本研究构建了RNA测序（RNA-Seq）文库，并通过HiSeq 2000测序仪获取了序列读取文件。