Next generation sequencing of human cholangiocarcinoma cells and associated stem-like component

The goal of this study is to provide a global transcriptome profiling (RNA-seq) of stem-like subset in human cholangiocarcinoma (CCA). Functional enrichment of CCA stem-like subset was performed by 3D sphere culture (SPH) in CCA cell lines. Comparison to parental CCA cells grown as 2D monolayer is provided. mRNA profile of 3D cholangio-spheres (SPH) compared to parental 2D monolayer (MON) cells of 4 different cholangiocarcinoma cell lines

本研究旨在获取人类胆管癌（cholangiocarcinoma, CCA）干细胞样亚群的全局转录组测序（RNA-seq）数据。针对该胆管癌干细胞样亚群的功能富集分析，通过在胆管癌细胞系中开展三维球体培养（SPH）完成，并以二维单层培养的亲本胆管癌细胞作为对照进行对比。本数据集包含4种不同胆管癌细胞系的三维胆管球状体（SPH）与对应亲本二维单层（MON）细胞的mRNA表达谱对比数据。