ada51201-2992-49f8-8298-f0d35db734ee - samples

Whole Exome sequencing data of tumour samples for 112 patients with endometrioid ovarian carcinoma in FASTQ format. Data was derived as summarized below:

Library Preparation: Libraries were prepared from each DNA sample using the Illumina TruSeq Exome Library Prep kit (#FC-150-1002) according to the provided protocol using modifications for working with FFPE sourced material. Libraries were quantified using the Qubit 2.0 Fluorometer and the Qubit DNA HS assay (#Q32854) and the size distribution of fragments was assessed using the Agilent Bioanalyser with the DNA HS Kit (#5067-4626).
Library QC: Exome-captured sequencing library pools were quantified using the Qubit 2.0 Fluorometer and the Qubit DNA HS assay (#Q32854) and the size distribution of fragments was assessed using the Agilent Bioanalyser with the DNA HS Kit (#5067-4626). Fragment size and quantity measurements were used to calculate molarity for each library pool.
Sequencing: Sequencing was performed using the NextSeq 500/550 High-Output v2 (150 cycle) Kit (# FC-404-2002) on the NextSeq 550 platform (Illumina Inc, #SY-415-1002).EGA dataset EGAD00001006389

本数据集包含112例子宫内膜样卵巢癌患者的肿瘤样本全外显子组测序（Whole Exome sequencing）数据，文件格式为FASTQ。数据制备流程概述如下：

文库构建：采用Illumina TruSeq外显子组文库制备试剂盒（货号#FC-150-1002），依照官方操作流程并针对福尔马林固定石蜡包埋（FFPE, Formalin-Fixed Paraffin-Embedded）样本进行适配修改后，对每份DNA样本构建测序文库。使用Qubit 2.0荧光计与Qubit DNA HS检测试剂盒（货号#Q32854）完成文库定量，并采用Agilent Bioanalyser与DNA HS试剂盒（货号#5067-4626）评估片段的尺寸分布。

文库质控：针对经过外显子组捕获的测序文库混合池，同样使用Qubit 2.0荧光计与Qubit DNA HS检测试剂盒（货号#Q32854）进行定量，并采用Agilent Bioanalyser与DNA HS试剂盒（货号#5067-4626）评估片段尺寸分布。结合片段长度与定量结果，计算各文库混合池的摩尔浓度。

测序：在NextSeq 550测序平台（Illumina公司，货号#SY-415-1002）上，使用NextSeq 500/550 High-Output v2（150循环）试剂盒（货号#FC-404-2002）完成测序。本数据集对应欧洲基因组-表型组学档案（EGA, European Genome-phenome Archive）数据集EGAD00001006389。