Induction of a thymus-independent immune response in human PBL engrafted NOD rag1?/? Prf1?/? mice.

PBL recipient mice were immunized with 20 ul/mouse pneumovax23 s.c. on the day of cell transfer. Serum was harvested on the day of sacrifice and assayed by ELISA for Streptococcus pneumoniae serotype 14 specific antibodies. Data representative of two experiments.细胞移植当日，对外周血淋巴细胞（Peripheral Blood Lymphocyte，PBL）受体小鼠以每只20 μL的剂量皮下注射Pneumovax23疫苗进行免疫。于安乐死当日采集血清，通过酶联免疫吸附试验（Enzyme-Linked Immunosorbent Assay，ELISA）检测血清中肺炎链球菌（Streptococcus pneumoniae）14血清型特异性抗体。本数据为两次独立实验的代表性结果。