A BAFF/APRIL axis regulates obesogenic-diet driven weight gain

Purpose: To define the role of the BAFF/APRIL axis in the regulation of adipose tissue homeostasis through RNAseq. Methods: Primary adipocytes were derived from pre-adipocyte precursors of inguinal white adipose from male C57BL/6 mice aged 6-8 weeks. Adipocytes were treated with rBAFF, rAPRIL, or multiple controls for 24 hours. mRNA profiles were generated for each condition, run in biological duplicates, using Illumina HiSeq2000 with single-end, 50bp reads. Differential gene lists were generated by comparing BAFF and APRIL to controls using unpaired ttests. Results:Following mapping an average of 30million reads across 10 samples to the mm10 mouse genome with annotations from UCSC, differential analysis identified genes involved in adipocyte pathways associated with lipid metabolism, lipolysis, and inflammation. Conclusion: BAFF and APRIL modulate modulate white adipocyte lipid handling. mRNA was obtained from inguinal adipose tissue obtained from male C57Bl/6 mice aged 6-8 weeks, following 24-hour treatment with rBAFF, rAPRIL, or multiple controls.

研究目的：通过RNA测序（RNAseq）阐明BAFF/APRIL轴在脂肪组织稳态调控中的作用。实验方法：从6-8周龄雄性C57BL/6小鼠腹股沟白色脂肪组织的前体脂肪细胞中分离原代脂肪细胞。将脂肪细胞分别用重组BAFF（rBAFF）、重组APRIL（rAPRIL）及多种对照试剂处理24小时。对每一组处理条件设置生物学重复样本，采用Illumina HiSeq2000平台进行单端50bp读长测序，生成各样本的mRNA表达谱。通过非配对t检验，将BAFF、APRIL处理组与对照组进行比较，得到差异基因列表。实验结果：将10个样本的平均3000万条读长序列比对至带有UCSC注释的mm10小鼠参考基因组后，差异表达分析筛选出了参与脂肪细胞脂质代谢、脂解及炎症相关通路的基因。研究结论：BAFF与APRIL可调控白色脂肪细胞的脂质代谢过程。本研究的mRNA样本取自6-8周龄雄性C57BL/6小鼠的腹股沟脂肪组织，经rBAFF、rAPRIL或多种对照试剂处理24小时后提取获得。