Oligonucleotide sequences used to construct 3 pSHK1393 and pSHK0945 plasmids.

aFinal constructs were used to replace endogenous sll1393 and sll0945 genes, respectively, by homologous recombination.
The position of the oligonucleotides in the nucleotide sequences of the sll1393 and sll0945 structural DNA are indicated. The restriction sites introduced by PCR amplification are underlined.

最终构建的重组载体分别通过同源重组技术，替换内源sll1393与sll0945基因。寡核苷酸（oligonucleotides）在sll1393和sll0945结构DNA的核苷酸序列中的位置已标注。经PCR扩增引入的限制性酶切位点已加下划线标注。