RNA-seq of P14 CD8 T cells during acute LCMV infection. RNA-seq of P14 CD8 T cells during acute LCMV infection

We performed RNA-seq, ATAC-seq, and H3K27Ac ChIP-seq on P14 CD8 T cells during acute infection with LCMV Armstrong on days 0 (naïve cells), 9 (memory precursor effector cells (MPEC) and short-lived effector cells (SLECs)), and 40+ (memory cells) post infection. Overall design: 4 samples (Naive, MPEC, SLEC, Memory), 2-3 biological replicates per sample

本研究针对感染淋巴细胞脉络丛脑膜炎病毒阿姆斯特丹株（LCMV Armstrong）的P14 CD8阳性T细胞，在其急性感染过程中的三个关键时间点——感染后第0天（初始T细胞）、第9天（记忆前体效应细胞（MPEC）与短寿效应细胞（SLECs））以及感染后40天及以上（记忆细胞）——分别开展了RNA测序（RNA-seq）、转座酶可及性测序（ATAC-seq）以及组蛋白H3赖氨酸27乙酰化染色质免疫共沉淀测序（H3K27Ac ChIP-seq）实验。实验整体设计：共设置4组样本（初始T细胞组、记忆前体效应细胞组、短寿效应细胞组、记忆细胞组），每组包含2至3次生物学重复。