Herpes viruses and human papilloma virus in nasal polyposis and controls

ABSTRACT INTRODUCTION: Chronic rhinosinusitis with nasal polyps is a multifactorial disease entity with an unclear pathogenesis. Contradictory data exist in the literature on the potential implication of viral elements in adult patients with chronic rhinosinusitis. OBJECTIVE: To compare the prevalence of human herpes viruses (1-6) and Human Papilloma Virus in adult patients with chronic rhinosinusitis with nasal polyps and healthy controls. METHODS: Viral DNA presence was evaluated by real-time polymerase chain reaction application to nasal polyps specimens from 91 chronic rhinosinusitis with nasal polyps patients and nasal turbinate mucosa from 38 healthy controls. RESULTS: Epstein-Barr virus positivity was higher in nasal polyps (24/91; 26.4%) versus controls (4/38; 10.5%), but the difference did not reach significance (p = 0.06). Human herpes virus-6 positivity was lower in nasal polyps (13/91; 14.29%) versus controls (10/38; 26.32%,p = 0.13). In chronic rhinosinusitis with nasal polyps group, 1 sample was herpes simplex virus-1-positive (1/91; 1.1%), and another was cytomegalovirus-positive (1/91; 1.1%), versus none in controls. No sample was positive for herpes simplex virus-2, varicella-zoster virus, high-risk-human papilloma viruses (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59) and low-risk-human papilloma viruses (6, 11). CONCLUSION: Differences in Epstein-Barr virus and human herpes virus-6 positivity among patients with chronic rhinosinusitis with nasal polyps and healthy controls are not statistically significant, weakening the likelihood of their implication in chronic rhinosinusitis with nasal polyps pathogenesis.引言：伴有鼻息肉的慢性鼻-鼻窦炎（chronic rhinosinusitis with nasal polyps, CRSwNP）是一种多因素疾病，其发病机制尚未明确。现有文献中关于病毒因子对成人慢性鼻-鼻窦炎患者的潜在影响存在矛盾结论。 研究目的：比较成人伴有鼻息肉的慢性鼻-鼻窦炎患者与健康对照者中人类疱疹病毒（human herpes viruses, HHV）1~6型及人乳头瘤病毒（Human Papilloma Virus, HPV）的检出率。 研究方法：采用实时聚合酶链反应（real-time polymerase chain reaction, qPCR），对91例伴有鼻息肉的慢性鼻-鼻窦炎患者的鼻息肉标本，以及38例健康对照者的鼻甲黏膜标本进行病毒DNA检测。 研究结果：鼻息肉组织中EB病毒（Epstein-Barr virus, EBV）阳性率为26.4%（24/91），高于对照组的10.5%（4/38），但差异未达到统计学显著性（p=0.06）。人类疱疹病毒6型阳性率在鼻息肉组为14.29%（13/91），低于对照组的26.32%（10/38），差异无统计学意义（p=0.13）。伴有鼻息肉的慢性鼻-鼻窦炎组中，1例样本单纯疱疹病毒1型阳性（1/91；1.1%），1例巨细胞病毒阳性（1/91；1.1%），对照组未检出上述病毒。所有样本均未检出单纯疱疹病毒2型、水痘-带状疱疹病毒、高危型人乳头瘤病毒（16、18、31、33、35、39、45、51、52、56、58、59型）及低危型人乳头瘤病毒（6、11型）。 研究结论：伴有鼻息肉的慢性鼻-鼻窦炎患者与健康对照者之间，EB病毒及人类疱疹病毒6型的阳性率差异无统计学显著性，提示二者在该病发病机制中发挥作用的可能性较低。