Gene expression analysis of Influenza vaccine response in Young and Old - Year 4. Homo sapiens

We profiled gene expression from a stratified cohort of subjects to define influenza vaccine response in Young and Old Overall design: Differential gene expression by human PBMCs from Healthy Adults receiving Influenza Vaccination (Fluvirin, Novartis). Healthy adults (older >65, younger 21-30 years) were recruited at seasonal Influenza Vaccination clinics organized by Yale University Health Services during October to December of 2013 – 2014 seasons. With informed consent, healthy individuals were recruited as per a protocol approved by Human Investigations Committee of the Yale University School of Medicine. Each subject was evaluated by a screening questionnaire determining self-reported demographic information, height, weight, medications and comorbid conditions. Participants with acute illness two weeks prior to vaccination were excluded from study. Blood samples were collected into BD Vacutainer Sodium Heparin tube at four different time points, once prior to administration of vaccine and three time points after vaccination on days 2, 7 and 28. Peripheral Blood Mononuclear Cells (PBMC) were isolated from heparinized blood using Histopaque 1077 in gradient centrifugation. About 1.0x10^7 freshly isolated PBMC were lysed in Triso and immediately stored in -80C. Total RNA in aqueous phase of Trisol - Chloroform was isolated in an automated QiaCube instrument using miRNeasy according to manufacturer’s instructions. Integrity of RNA samples were assessed by Agilent 2100 BioAnalyser Samples were processed for cRNA generation using Illumina TotalPrep cRNA Amplification Kit and subsequently hybridized to Human HT12-V4.0 BeadChip at Yale Center for Genomic Analysis (YGCA). The current data set, together with GSE59654, GSE59635, GSE59743, and GSE101710, represents subsets of the same overall study

本研究对分层招募的受试者队列进行基因表达谱分析，以明确青年与老年群体的流感疫苗应答特征。
整体实验设计：针对接种流感疫苗（诺华公司Fluvirin疫苗）的健康成年人的人外周血单个核细胞（Peripheral Blood Mononuclear Cells，PBMC）开展差异基因表达分析。
研究于2013-2014流感季的10月至12月期间，在耶鲁大学健康服务中心组织的季节性流感疫苗接种门诊招募健康成年人受试者，分为老年组（年龄>65岁）与青年组（年龄21-30岁）。所有受试者均签署知情同意书，招募流程符合耶鲁大学医学院人类研究委员会批准的实验方案。通过筛查问卷收集每位受试者的自我报告人口学信息、身高、体重、用药情况及合并症状况。接种疫苗前两周内患有急性疾病的受试者被排除出本研究。
在四个时间点采集血液样本：疫苗接种前1次，接种后第2、7、28天各采集1次，血液样本采集于BD Vacutainer Sodium Heparin tube中。采用Histopaque 1077梯度离心法从肝素抗凝血液中分离外周血单个核细胞（PBMC）。将约1.0×10^7个新鲜分离的PBMC在Triso试剂中裂解后，立即置于-80℃冰箱保存。采用TRIzol-氯仿法提取水相中的总RNA，使用自动化QiaCube仪器并参照miRNeasy试剂盒制造商的操作指南进行纯化。使用安捷伦2100生物分析仪（Agilent 2100 BioAnalyser）评估RNA样本的完整性。使用Illumina TotalPrep cRNA扩增试剂盒制备cRNA，随后在耶鲁大学基因组分析中心（YGCA）将样本杂交至人类HT12-V4.0微珠芯片（Human HT12-V4.0 BeadChip）。
本数据集与GSE59654、GSE59635、GSE59743及GSE101710共同构成该整体研究的多个子数据集。