Analyzed mass spec data.

Epigenetic regulation and metabolism are connected. Epigenetic regulators, like the SIN3 complex, affect the expression of a wide range of genes, including those encoding metabolic enzymes essential for central carbon metabolism. The idea that epigenetic modifiers can sense and respond to metabolic flux by regulating gene expression has long been proposed. In support of this cross-talk, we provide data linking SIN3 regulatory action on a subset of metabolic genes with the cellular response to changes in metabolic flux. Furthermore, we show that loss of SIN3 is linked to decreases in mitochondrial respiration and the cellular response to mitochondrial and glycolytic stress. Data presented here provide evidence that SIN3 is important for the cellular response to metabolic flux change.表观遗传调控（Epigenetic regulation）与新陈代谢（metabolism）存在密切关联。诸如SIN3复合物（SIN3 complex）这类表观遗传调控因子，能够调控广泛的基因表达，其中包括编码参与中心碳代谢（central carbon metabolism）所必需的代谢酶的基因。长期以来，学界已提出表观遗传修饰因子（epigenetic modifiers）可通过调控基因表达，感知并响应代谢流（metabolic flux）的假说。为验证这一表观遗传与代谢的串扰现象，本研究提供了数据，表明SIN3对部分代谢基因的调控作用与细胞对代谢流变化的响应存在关联。此外，本研究还证实，SIN3的缺失会导致线粒体呼吸作用（mitochondrial respiration）水平下降，并影响细胞对线粒体应激与糖酵解应激（glycolytic stress）的响应。本文所呈现的数据证明，SIN3在细胞响应代谢流变化的过程中发挥着关键作用。