Microlag - Datasets & Scripts

In this archive, the reader will find curated datasets, scripts to analyze them and produced figures. Abstract: Young oysters of Pacific oyster Magallana (Crassostrea) gigas are regularly impacted by summer mortalities since 2008, caused by the infection by the Ostreid herpesvirus 1 (OsHV-1) when seawater temperature increases in summer. Adult oysters rather suffer from a vibriosis caused by the bacterial primary pathogen Vibrio aestuarianus francensis. Although these diseases are now well described, little is known about their contribution and possible synergy in natural mortality events of juvenile oysters. Using an in-situ approach conducted in the Thau lagoon, we investigated the potential implication of Vibrio aestuarianus in the juvenile oyster’s mortality, as well as the virus OsHV-1, throughout a large sampling period from winter to late summer, after the mortality event. We further determined their respective effects on the microbial abundance and diversity on alive and moribund oysters. We demonstrated that microbiomes are dynamic throughout seasons and tend to homogenize in spring with a dominance of Mycoplasmataceae in alive oysters. Surprisingly, our data highlighted the prevalence and high loads of Vibrio aestuarianus in all moribund oysters. Contrasting with literature, we found that the microbial communities of moribund juvenile oysters were significantly influenced by both Vibrio aestuarianus and the virus OsHV-1. The moribund bacterial indicators were detected in abundance during mortalities in the surrounding environment of oyster farms and further (700m far). These results highlight the connectivity between the different compartments of the lagoon and raise questions about the potential spread of the primary pathogen Vibrio aestuarianus from adult to juvenile oysters in farming areas located in lagoons.本归档文件包含经筛选整理的数据集、用于数据分析的脚本以及生成的实验图表。 摘要： 自2008年起，太平洋牡蛎（Pacific oyster，*Magallana (Crassostrea)* gigas）的幼贝频繁暴发夏季大规模死亡事件，夏季海水升温时，其会被牡蛎疱疹病毒1型（Ostreid herpesvirus 1, OsHV-1）感染致病。而成体牡蛎则常罹患由优势病原菌河口弧菌法国亚种（Vibrio aestuarianus francensis）引发的弧菌病。尽管目前学界已对这两类病害有较为充分的描述，但对于二者在太平洋牡蛎幼贝自然死亡事件中的具体贡献及潜在协同效应，目前仍知之甚少。 本研究依托塔乌泻湖（Thau lagoon）开展原位实验，在一次大规模死亡事件发生后，于冬季至夏末的长周期采样过程中，探究了河口弧菌法国亚种与牡蛎疱疹病毒1型在幼贝死亡事件中的潜在作用。本研究进一步明确了两种病原对存活与濒死牡蛎的微生物丰度及群落多样性的各自影响。 研究结果显示，牡蛎微生物组随季节呈现动态变化趋势，春季时群落结构趋于均质化，存活牡蛎的微生物组以柔膜菌科（Mycoplasmataceae）为优势类群。令人意外的是，本研究数据显示，所有濒死牡蛎体内均检测到高载量的河口弧菌法国亚种，且该菌呈现高流行率。与已有文献结论不同的是，本研究发现濒死幼贝的微生物群落同时受到河口弧菌法国亚种与牡蛎疱疹病毒1型的显著调控。在牡蛎养殖场周边环境乃至700米外的水域中，均能检测到与濒死牡蛎相关的高丰度指示菌群，且在死亡事件高发期其丰度显著升高。本研究结果揭示了泻湖不同生态单元间的连通性，并对泻湖养殖区中优势病原菌河口弧菌法国亚种从成体牡蛎向幼贝的潜在传播路径提出了新的科学问题。