RNA sequencing (RNA-seq) of PQBP1 knockdown in ovarian cancer cells

To investigate the comprehensive function of PQBP1 in the regulation of RNA splicing, we established Dox-inducible PQBP1 knockdown HEY cell line. We then performed gene expression profiling analysis using data obtained from RNA-seq of PQBP1 knockdown and control HEY cells (three biological replications of each sample). Overall design: Comparative gene expression profiling analysis of RNA-seq data for Dox-inducible PQBP1 knockdown HEY cells (shNC, shPQBP1). The assay was performed on three biological triplicates.

为探究PQBP1在RNA剪接调控中的全面功能，我们构建了多西环素（Dox）诱导型PQBP1敲低HEY细胞系。随后采用PQBP1敲低组与对照组HEY细胞的RNA测序（RNA-seq）数据开展基因表达谱分析，每组设置三次生物学重复。实验整体设计：针对多西环素诱导型PQBP1敲低HEY细胞（分为阴性对照shNC组、PQBP1敲低shPQBP1组）的RNA测序数据进行对比基因表达谱分析，全部实验均完成三次生物学重复。