Expression data from rat hippocampus with chronic intermittent hypoxia exposure

Obstructive sleep apnea syndrome (OSA) is a common sleep disorder. OSA is an independent risk factor for cognitive impairment. Chronic intermittent hypoxia (CIH) is the main characteristic of OSA and the main potential culprit of OSA-induced hippocampal damage. The mechanism of CIH in the development of cognitive impairment remains unclear The microarray was used to investigate the differentially expressed long non-coding RNAs(lncRNAs), microRNAs (miRNAs) and mRNAs in the hippocampus of control and CIH-exposed rats. The rats in control group and CIH group were exposed to normoxia for 4 weeks and CIH for 4 weeks, respectively. Overall design: Examine the hippocampus of control and CIH-exposed rats. For RNA-seq, the total RNA was harvested using TRIzol reagent (Invitrogen, Carlsbad, CA, USA). The amount and quality of RNA were determined using a NanoAnalyzer and Agilent 2100 biological analyzer (Thermo Fisher Scientific, Ma, USA). The mRNA expression profiling was measured using BGISEQ-500/ MGISEQ-2000system (BGI Co., Shenzhen, China).

阻塞性睡眠呼吸暂停综合征（Obstructive sleep apnea syndrome, OSA）是一种常见的睡眠障碍。OSA是引发认知功能损害的独立危险因素。慢性间歇性缺氧（Chronic intermittent hypoxia, CIH）是OSA的核心病理特征，也是OSA诱导海马损伤的主要潜在致病因素。目前CIH介导认知功能损害的具体分子机制仍未明确。本研究采用微阵列芯片技术，分析对照组与CIH暴露大鼠海马组织中差异表达的长链非编码RNA（long non-coding RNAs, lncRNAs）、微小RNA（microRNAs, miRNAs）及信使RNA（messenger RNAs, mRNAs）的表达谱。对照组大鼠暴露于常氧环境4周，CIH组大鼠则接受慢性间歇性缺氧处理4周。实验整体设计：对对照组与CIH暴露大鼠的海马组织进行检测分析。在RNA测序相关实验中，总RNA采用TRIzol试剂（Invitrogen, 美国加利福尼亚州卡尔斯巴德市）提取；RNA的浓度与质量通过NanoAnalyzer分析仪与Agilent 2100生物分析仪（赛默飞世尔科技公司，美国马萨诸塞州）进行检测。mRNA表达谱测序采用BGISEQ-500/MGISEQ-2000测序平台（深圳华大基因股份有限公司，中国深圳）完成。