Transcriptomic responses of the liver and adipose tissues to altered carbohydrate-fat ratio in diet: An isoenergetic study in young rats

To elucidate the effects of altered dietary carbohydrate and fat balance on liver and adipose tissue transcriptomes, 3-week-old rats were fed three kind of diets: low, moderate and high fat diets (L, M and H) containing a different ratio of carbohydrate:fat (C:F) (65:15, 60:20 and 35:45 in energy percent, respectively). Rats consumed the diets for 9 weeks, and were subjected to biochemical and DNA -microarray analyses.

为阐明膳食碳水化合物与脂肪比例变化对肝脏及脂肪组织转录组（transcriptome）的影响，本研究选取3周龄大鼠，饲喂三种不同膳食：低脂（L）、中脂（M）与高脂（H）膳食，三者的碳水化合物:脂肪（C:F）能量占比各异，分别为65:15、60:20及35:45。大鼠持续饲喂上述膳食9周后，接受生化检测与DNA微阵列（DNA-microarray）分析。