CIL:10426, Gallus gallus, erythrocyte. In Cell Image Library

Nucleated erythrocytes from chicken were spread on water, the dispersed chromatin picked up on carbon-formvar grids, fixed with paraformaldehye, critical point dried and shadowed with platinum. Images were obtained with the Wisconsin high voltage TEM at 1MEV using darkfield illumination. For this micrograph, the grid was tilted to 55 degrees. A similar micrograph tilted to 44 degrees providing a stereo pair with an oblique 3D view of the tangled and irregular chromatin fibers is grouped with this image. The combination of shadowing and darkfield illumination gives a quasi-negative stain effect revealing nucleosomes in some fibers.

将鸡有核红细胞铺展于水面，使分散的染色质吸附于碳-福尔瓦（carbon-formvar）复合电镜载网，随后经多聚甲醛固定、临界点干燥及铂喷镀处理。图像采集使用威斯康星大学1兆电子伏特高压透射电子显微镜，采用暗场照明模式。本显微图像对应的载网倾角为55度；另有一张载网倾角为44度的配套显微图像，二者组成立体图像对，可呈现缠绕紊乱、形态不规则的染色质纤维的倾斜三维视图，该图像与本图像归为一组。喷镀与暗场照明的联用可产生类负染效果，使部分纤维中的核小体（nucleosomes）结构得以清晰显现。