Additional file 1 of Causal association between low vitamin D and polycystic ovary syndrome: a bidirectional mendelian randomization study

Additional file 1: Table S1. STROBE-MR Checklist; Table S2. Key characteristics of participating studies; Table S3. GWAS significant SNPs used as genetic instruments for VD level on PCOS; Table S4. GWAS significant SNPs used as genetic instruments for PCOS on VD level; Table S5. GWAS significant SNPs used as genetic instruments for VD level on BT; Table S6. GWAS significant SNPs used as genetic instruments for BT on PCOS; Table S7. GWAS significant SNPs used as genetic instruments for BT and VD level on PCOS; Table S8. Heterogeneity and directional pleiotropy test using MR-Egger intercepts; Table S9. Potentially relevant genes corresponding to IVs associated with VD and PCOS; Table S10. Potentially relevant genes corresponding to IVs associated with VD and PCOS; Table S11. GO and KEGG enrichment analysis for potentially relevant genes related to VD and PCOS; Table S12. GO and KEGG enrichment analysis for potentially relevant genes related to VD and BT; Figure S1. Scatter plot of the MR estimates for the association of VD level with PCOS; Figure S2. Funnel plot reveals overall heterogeneity of the impact of VD on PCOS; Figure S3. Leave-one-out analysis of the impact of the VD on PCOS.

附加文件1：表S1：STROBE-MR检查表；表S2：纳入研究的关键特征；表S3：用作多囊卵巢综合征（Polycystic Ovary Syndrome，PCOS）维生素D（Vitamin D，VD）水平遗传工具的全基因组关联研究（Genome-Wide Association Study，GWAS）显著单核苷酸多态性（Single Nucleotide Polymorphisms，SNPs）；表S4：用作维生素D（VD）水平对多囊卵巢综合征（PCOS）影响的遗传工具的GWAS显著SNPs；表S5：用作BT表型中维生素D（VD）水平遗传工具的GWAS显著SNPs；表S6：用作多囊卵巢综合征（PCOS）中BT表型遗传工具的GWAS显著SNPs；表S7：用作多囊卵巢综合征（PCOS）中BT表型与维生素D（VD）水平共同遗传工具的GWAS显著SNPs；表S8：采用孟德尔随机化（Mendelian Randomization，MR）-Egger截距开展异质性与方向性多效性检验；表S9：与维生素D（VD）和多囊卵巢综合征（PCOS）相关的工具变量（Instrumental Variables，IVs）所对应的潜在相关基因；表S10：与维生素D（VD）和多囊卵巢综合征（PCOS）相关的工具变量（IVs）所对应的潜在相关基因；表S11：针对与维生素D（VD）和多囊卵巢综合征（PCOS）相关的潜在相关基因的基因本体论（Gene Ontology，GO）与京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes，KEGG）富集分析；表S12：针对与维生素D（VD）和BT相关的潜在相关基因的GO与KEGG富集分析；图S1：维生素D（VD）水平与多囊卵巢综合征（PCOS）关联的MR估计值散点图；图S2：漏斗图揭示维生素D（VD）对多囊卵巢综合征（PCOS）影响的总体异质性；图S3：维生素D（VD）对多囊卵巢综合征（PCOS）影响的留一法分析。