Comparative transcriptional analysis of Desulfovibrio alaskensis G20, a choline utilization regulator and LysX

Expression data for Desulfovibrio alaskensis strain G20 and mutants in regulator proteins grown on lactate sulfate media and then pelleted and transferred to another media when they reached stationary phase. The Choline mutant was transferred to lacte/sulfate minimal media and choline/sulfate minimal media. The LysX mutant was transferred to minimal media with lysine and rich media. G20 was transferred to minimal media, choline/sulfate minimal media, lactate/choline/sulfate minimal media, minimal media with lysine, and rich media. We aimed to confirm or expand the regulons of each of the transposon interupted regulator mutants and compare gene expression responses of the regulators in different growth conditions. 10 samples were collected: 2 regulator mutants (2 conditions each), Desulfovibrio alaskensis G20 (5 conditions), 2 replicates for G20 minimal media condition. Control sample -G20 rich media.

本数据集收录阿拉斯脱硫弧菌（Desulfovibrio alaskensis）G20野生型菌株及其转座子插入失活的调控蛋白突变株的基因表达数据。所有菌株均先于乳酸盐-硫酸盐培养基中培养至稳定期，收集菌体后转接至不同的后续培养基。其中，胆碱（choline）突变株分别转接至乳酸盐/硫酸盐基本培养基与胆碱/硫酸盐基本培养基；LysX突变株分别转接至含赖氨酸的基本培养基与丰富培养基；野生型G20菌株则分别转接至5种培养基：基础基本培养基、胆碱/硫酸盐基本培养基、乳酸盐/胆碱/硫酸盐基本培养基、含赖氨酸的基本培养基以及丰富培养基。本研究旨在验证或拓展每一株转座子插入失活的调控突变株的调控子（regulon），并比较不同生长条件下各调控因子的基因表达响应特征。本次实验共收集10份样本：2株调控因子突变株各对应2种培养条件（各1次生物学重复），野生型G20菌株的5种培养条件中，基础基本培养基设置2次生物学重复，其余条件各1次生物学重复。其中野生型G20的丰富培养基培养样本为实验对照样本。